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Bill Wolfe, D.D.S., N.M.D.

Bactericidal Studies

Various percentages of Aloe Vera gels were tested in culture media consisting of the following organisms: Staphylococcus aureus, Streptococcus viridans, Candida albicans, Corynebacterium xerosis, and the five strains of Streptococcus mutans most commonly found in dental plaque. The gel was bactericidal against the above bacteria and fungicidal against Candida albicans (the cause of moniliasis or 'denture sore mouth.') An important finding by Zimmerman was that the dramatic effects of the aloe gel were not apparent until there was at least a 70% concentration of the gel. To achieve a 70% or greater concentration, an Aloe Vera gel must be stabilized with anti-oxidants in order to minimize the otherwise necessary preservatives which lessen the percentages of Aloe Vera in the product.

Virucidal Studies

The Aloe Vera gel formula was found to be virucidal against Herpes simplex and Herpes zoster viruses.

Anti-inflammatory Studies

Prednisolone and Indomethacin (common anti-inflammatory drugs) were utilized in a comparative study with the Aloe Vera gel to observe the effects on tissue culture cells. The gel was found to be just as effective as Prednisolone and Indomethacin, without having the long term toxicity of either drug.

Cell Cytotoxicity Studies

Human embryonic kidney cells (HEK cells) were utilized to determine the effectiveness of aloe gel on cellular longevity. The cellular death rate was found to be reduced by 2/3 when cultured with the aloe gel. Note: It is important to acknowledge research by Dr. Wendell Winters, Associate Professor of Microbiology at the University of Texas Health Science Center, San Antonio, Texas. Dr. Winters found that although Aloe Vera promoted the growth and healing of healthy human cells, the gel did not have the unwanted effect of stimulating the growth of cancer cells.

Dr. Zimmerman's studies proved the Aloe Vera gel formula to be nontoxic, bactericidal, virucidal, and fungicidal against a broad range of micro-organisms, and a stimulator of cellular life-extension. This research and the clinical effects which I had witnessed in my office resulted in a commitment on my part to discover the uses of the aloe gel in all aspects of dental health. Now that I had laboratory and clinical evidence of the effectiveness and biocompatibility of my Aloe Vera formula, I felt that the next step for oral health should be to develop an Aloe Vera-based toothpaste.

Because a tooth paste or a tooth gel must include abrasives (of which examples are listed below), a surface active agent (foaming agent), a sweetener to counter the taste of the various ingredients (including the pungent taste of aloe gel), and a breath freshener, I was concerned about how much the healthful properties of the gel might be compromised by being combined in such a formula.

The bactericidal effects on dental plaque are a primary concern in evaluation and measuring the effectiveness of a toothpaste. Such bactericidal potency can be measured in the laboratory by means of a 'sensitivity disc' method of analysis. This method utilizes agar plates ("Petri" dishes) upon which are grown bacterial cultures of the various micro-organisms to be tested. The next project tested the Aloe Dent toothpaste formula against the various bacterial components of dental plaque (LM-7, BHT, AHT, 6715, and GS-5 strains of Streptococcus mutans bacteria.) After two hours of incubation, sterile paper discs were placed on the inoculated dishes. On each disc 0.3 grams of sample toothpaste was used in each experiment.

Each dish was examined after 24 and 48 hours to detect zones of clearing for inhibition (kill) of the Streptococcus mutans bacteria. The measurement from the edge of the disc to the end of the clear zone surrounding the disc indicates the depth of 'kill' of the bacteria, i.e., the larger the number of millimeters, the more potent the bactericidal effects. The chart below demonstrates the results after 48 hours of bactericidal analysis, comparing the Aloe-Dent™ formula to Crest® and Colgate® toothpastes.

This chart demonstrates the bactericidal effectiveness of Aloe-Dent™ against the 5 strains of Streptococcus mutans bacteria in dental plaque. The fact that the Aloe-Dent™ range was greater than that of either Crest® or Colgate® is very noteworthy, as the bactericidal results from the Crest® and Colgate® testing are due to fluoride. Aloe-Dent™ was able to achieve equal or better results without the addition of fluoride, which is controversial due to its wide range of potentially harmful systemic effects.

Conclusion

The previous research is only a partial indication of the potential benefits which Aloe Vera can provide for dentistry. I encourage health professionals to investigate, experiment, and conclude for themselves as to the effectiveness, new uses, and exciting future which Aloe Vera has in dentistry.

References:

1) Kent CM. Aloe Vera. Arlington VA. 1979:14.
2) Goats BC. The Silent Healer: A Modern Study of Aloe Vera. Garland, TX, 1979.
3) Sims RM, Zimmerman ER. Report on effect of aloe vera on growth of certain micro-organisms. Baylor College of Dentistry, Dallas Microbial Assay Services. Ava Inc Archives. 1969; Vol 1:230-233.
4) El Zawahr M, Hegazy RM, Helal M. Uses of aloe in treating leg ulcers and dermatosis. Dermatology (1973 Jan-Feb):72.
5) Crewe J. The external use of aloe. Minn J of Med. (1937 Oct) 20 (538-539.)
6) Collins E. Alvagel as a therapeutic agent in treatment of Roentgen and radiation burns. Radiological Review and Chicago Medical Recorder. (1935 June) 57:137-138.
7) Wright CS. Aloe vera in the treatment of roentgen ulcers and telangiectasis. JAMA. 1936 (106):1363-1364.
8) Lushbaugh CC, Hale DB. Experimental acute radiodermatitis following beta irradiation. Cancer.
1953:698.
9) Brasher JW, Collings CK, Zimmerman ER. The effects of prednisolone, indomethacin and aloe vera gel on tissue culture cells. Oral Surgery, Oral Medicine, and Oral Pathology. 1969.27 (1): 122-128.
10) Bovik EG. Aloe vera: Panacea or old wives tale? Texas Dental J. 1966:15.
11) Rowe TD, Parks LM, Lovell BK. Further observations on the use of aloe vera leaf in the treatment of third degree x-ray reaction. JAMA. 1941:270.

Fluoride toxicity